Enhanced Fluorescence - Label Technology
ComBIND™ Microarray Slide
ComBIND™, exclusively licensed from Novartis for drug discovery, is a novel luminescent technology
with superior sensitivity for microarray and microtiter plate applications. The ComBIND technology
leverages SRU Biosystems’ expertise in developing and manufacturing novel surfaces on nanostructured
layers. ComBIND sensors can be manufactured to work with virtually any labeling technology. The
enhanced sensitivity of ComBIND is due to an increase in the efficiency of excitation of surface-bound
fluorophores in the evanescent field of the sensor surface (see reference below). In one
representative assay the fluorescent yield was increased more than 20-fold, even in the presence of
serum, when compared to conventional fluorescent microarray slides (see Figure 1 A and B). Improvements
in the lower limit of detection (LLD) of greater than 10-fold are observed. ComBIND slides are
compatible with commercially available laser scanners, confocal microscopes and miniaturized CCD
read-out equipment.
SRU Biosystems now offers Cy-5 compatible ComBIND microarray slides for commercial sale. For ordering information contact info@srubiosystems.com.
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Figure 1A & B: Detection of IL-6 in serum on ComBIND microarray slides. The IL-6 sandwich assay on glass or ComBIND slides using a Cy5 labeled secondary antibody. On ComBIND 100fmols/ml of IL6 in serum can be detected. Tecan Laser Scanner used to read the slide.
SRU Biosystems now offers Cy-5 compatible ComBIND microarray slides for commercial sale. For ordering information contact info@srubiosystems.com.
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Click to enlarge
Figure 1A & B: Detection of IL-6 in serum on ComBIND microarray slides. The IL-6 sandwich assay on glass or ComBIND slides using a Cy5 labeled secondary antibody. On ComBIND 100fmols/ml of IL6 in serum can be detected. Tecan Laser Scanner used to read the slide.
Reference: Neuschafer et al (2003) Biosensors & Bioelectronics 18, 489-497