SRU Biosystems - BIND Reader >> SCREENER

BIND Readers >> SCREENER

The BIND® SCREENER is label-free, plate-based reader designed for ultra-high throughput environments and provides quantitative measurements of a variety of cellular and biochemical interactions. The SCREENER is a small footprint, bench-top instrument capable of reading 96-, 384-, 384LV- and 1536-well Biosensor plates. It is easily integrated within a variety of automation platforms. Throughput can be maximized using the TURBO read mode option which allows a 1536-well plate to be read in as little as 45 seconds.

SCREENER Features

96-, 384- and 1536-well read capabilities
Ideal for in vitro biochemical and live cell-based assays
Flexible automation integration, including on-board liquid handling
Unmatched label-free read times
Kinetic & endpoint measurements produce information-rich data
Real-time data acquisition & viewing

Highly Reliable & Reproducible Data

A. Day-to-Day Reproducibility	C. Plate-to-Plate Reproducibility

Agonist	pEC₅₀	Z’ achieved	Agonist	pEC₅₀	Z’ achieved
Acetylcholine	Day 1: 7.8 Day 2: 8.0	0.93	Acetylcholine	Plate 1: 8.0 Plate 2: 8.1	0.87
Carbachol	Day 1: 7.0 Day 2: 7.1	0.90	Carbachol	Plate 1: 6.9 Plate 2: 7.1	0.83

Figure 1 - Cells expressing the M4 muscarinic receptor were plated onto BIND® CA-2 Biosensors. Acetylcholine or carbachol were added using the Biomek® Fx-BIND SCREENER integration platform and responses measured. Graphs show concentration curves of two different 384-well plates run on two different days (A) or two different 1536-well plates run the same day (B), demonstrating the well-to-well, plate-to-plate and day-to-day reproducibility of BIND.

The SCREENER is a highly scalable instrument that is flexible enough for assay development and powerful enough for high throughput screening. Assays developed in 384-well formats can be easily migrated to 1536-well formats enabling miniaturization and downstream screening without sacrificing assay performance. Using BIND’s sensitive and robust technology, the SCREENER produces superior results for miniaturized, biochemical and cell-based assays. Z factors are routinely above 0.8 with CVs <10% as shown in Figure 1 for a 384/1536-well GPCR activation assay.

Flexible Automation Integration Solutions

The SCREENER’s small footprint, easy-access plate holder and open architecture software enable simplified integration within customers’ preferred automation solution. BIND Readers have successfully been integrated with industry-standard laboratory automation devices including robotic arms, liquid handling systems and plate hotels. Ultra-high throughput speeds are achieved with a SCREENER-Biomek® FX integration package that can assay over 120,000 samples per 8 hour day in a 1536-well Biosensor format.

Unmatched Detection Times Using SRU’s TURBO Read Mode

The SCREENER is available with an optional TURBO read mode that decreases read times by up to 75%. Using TURBO, a 384-well BIND Biosensor can be read in <20 seconds and a 1536-well Biosensor in <60 seconds. The TURBO option is available at the time of purchase or as a field upgrade.

SRU’s EMS Software: Powerful User-Interface

All BIND Readers are supplied with SRU’s Experiment Management System (EMS) software. EMS provides complete control of assay parameters through an easy-to-use, intuitive interface and allows for visualization, export and secure storage of all data. Data can be exported from EMS using a custom export wizard in several file formats for maximum flexibility.

EMS User Interface

EMS Data Export Wizard

SCREENER Specifications

Compatible plates:	All 96-, 384-, 384-LV and 1536-well BIND® Biosensor microplates
Read Time:	96-well in 30 seconds, 384-well in 1 minute, 1536-well in 3 minutes
TURBO Read Times:	96-well in 8 seconds, 384-well in 15 seconds, 1536-well in 45 seconds
Operating Temperature Range:	4°C to 37°C
Dimensions:	15.5 in. (W) x 17.6 (D) x 14 in. (H)
Weight:	68 lbs.
Computer:	Computer and monitor included